An Evaluation of the Faster STORM Method for Super-resolution Microscopy
2014 (English)In: Proceedings of the 22nd International Conference on Pattern Recognition, 2014, 4435-4440 p.Conference paper (Refereed)
Development of new stochastic super-resolution methods together with fluorescence microscopy imaging enables visualization of biological processes at increasing spatial and temporal resolution. Quantitative evaluation of such imaging experiments call for computational analysis methods that localize the signals with high precision and recall. Furthermore, it is desirable that the methods are fast and possible to parallelize so that the ever increasing amounts of collected data can be handled in an efficient way. We here in address signal detection in super-resolution microscopy by approaches based on compressed sensing. We describe how a previously published approach can be parallelized, reducing processing time at least four times. We also evaluate the effect of a greedy optimization approach on signal recovery at high noise and molecule density. Furthermore, our evaluation reveals how previously published compressed sensing algorithms have a performance that degrades to that of a random signal detector at high molecule density. Finally, we show the approximation of the imaging system's point spread function affects recall and precision of signal detection, illustrating the importance of parameter optimization. We evaluate the methods on synthetic data with varying signal to noise ratio and increasing molecular density, and visualize performance on realsuper-resolution microscopy data from a time-lapse sequence of livingcells.
Place, publisher, year, edition, pages
2014. 4435-4440 p.
, International Conference on Pattern Recognition, ISSN 1051-4651
Signal Processing Medical Image Processing
Research subject Computerized Image Processing
IdentifiersURN: urn:nbn:se:uu:diva-238600DOI: 10.1109/ICPR.2014.759ISI: 000359818004096ISBN: 978-1-4799-5208-3OAI: oai:DiVA.org:uu-238600DiVA: diva2:771539
22nd International Conference on Pattern Recognition, 24-28 August, 2014S, tockholm, Sweden