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Inhibition of Mammalian Target of Rapamycin Induces Renal Mitochondrial Uncoupling in Rats
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
2013 (English)In: Oxygen Transport To Tissue XXXV, 2013, 309-314 p.Conference paper, Published paper (Refereed)
Abstract [en]

The mechanisms underlying diabetic nephropathy are not fully understood. However, recent research indicates mitochondria dysfunction as a contributing factor. Mammalian target of rapamycin (mTOR) is a known regulator of mitochondria function and could therefore also be involved in the development of diabetic nephropathy. The present study investigates the role of mTOR for controlling the function of mitochondria isolated from normal and diabetic rat kidneys. Control and streptozotocin-induced diabetic rats were treated with the mTOR inhibitor rapamycin (0.2 mg/day) by oral gavage for 14 days, after which mitochondria function was investigated using high-resolution respirometry. Mitochondrial uncoupling was defined as increased oxygen usage unrelated to ATP production. mTOR inhibition induced mitochondria uncoupling in control rats, but did not affect the already occurring uncoupling in kidney mitochondria from diabetic animals. Inhibition of mTOR using rapamycin induces mitochondria uncoupling in control rats, suggesting a role of mTOR as a moderator of mitochondria efficiency. No effect of mTOR inhibition was observed in mitochondria from diabetic animals, suggesting that there are other pathways in addition to the mTOR pathway regulating mitochondria function in diabetes. The functional significance of the mTOR pathway in regulating mitochondria efficiency warrants further attention.

Place, publisher, year, edition, pages
2013. 309-314 p.
Series
Advances in Experimental Medicine and Biology, ISSN 0065-2598 ; 789
National Category
Physiology
Identifiers
URN: urn:nbn:se:uu:diva-238101DOI: 10.1007/978-1-4614-7411-1_41ISI: 000343662700042ISBN: 978-1-4614-7411-1; 978-1-4614-7256-8 (print)OAI: oai:DiVA.org:uu-238101DiVA: diva2:771763
Conference
40th Annual Meeting of the International-Society-on-Oxygen-Transport-to-Tissue (ISOTT), AUG 19-24, 2012, Bruges, BELGIUM
Available from: 2014-12-15 Created: 2014-12-09 Last updated: 2014-12-15Bibliographically approved

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Sivertsson, EbbaFriederich-Persson, Malou

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