Antigen Transfer from Exosomes to Dendritic Cells as an Explanation for the Immune Enhancement Seen by IgE Immune Complexes
2014 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 9, no 10, e110609- p.Article in journal (Refereed) Published
IgE antigen complexes induce increased specific T cell proliferation and increased specific IgG production. Immediately after immunization, CD23(+) B cells capture IgE antigen complexes, transport them to the spleen where, via unknown mechanisms, dendritic cells capture the antigen and present it to T cells. CD23, the low affinity IgE receptor, binds IgE antigen complexes and internalizes them. In this study, we show that these complexes are processed onto B-cell derived exosomes (bexosomes) in a CD23 dependent manner. The bexosomes carry CD23, IgE and MHC II and stimulate antigen specific T-cell proliferation in vitro. When IgE antigen complex stimulated bexosomes are incubated with dendritic cells, dendritic cells induce specific T-cell proliferation in vivo, similar to IgE antigen complexes. This suggests that bexosomes can provide the essential transfer mechanism for IgE antigen complexes from B cells to dendritic cells.
Place, publisher, year, edition, pages
2014. Vol. 9, no 10, e110609- p.
Immunology in the medical area Microbiology in the medical area
IdentifiersURN: urn:nbn:se:uu:diva-238568DOI: 10.1371/journal.pone.0110609ISI: 000343942100076PubMedID: 25330118OAI: oai:DiVA.org:uu-238568DiVA: diva2:772470