uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Subgroup-dependent effects of voluntary alcohol intake on behavioral profiles in outbred Wistar rats
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
2014 (English)In: Behavioural Brain Research, ISSN 0166-4328, E-ISSN 1872-7549, Vol. 275, 288-296 p.Article in journal (Refereed) Published
Abstract [en]

Experimental animal models are critical for understanding the genetic, environmental and neurobiological underpinnings of alcohol use disorders. Limited studies investigate alcohol-induced effects on behavior using free-choice paradigms. The aims of the present experiment were to study voluntary alcohol intake using a modified intermittent access paradigm, investigate the effects of voluntary alcohol intake on behavioral profiles in water- and alcohol-drinking rats, and select extreme low- and high-drinking animals for a more detailed behavioral characterization. Sixty outbred male Wistar rats were randomized into water and alcohol groups. Behavioral profiles in the multivariate concentric square field (TM) (MCSF) test were assessed prior to and after voluntary alcohol intake. The animals had intermittent access to 20% alcohol and water for three consecutive days per week for seven weeks. The results revealed increased alcohol intake over time. No major alcohol-induced differences on behavior profiles were found when comparing water- and alcohol-drinking animals. The high-drinking animals displayed an alcohol deprivation effect, which was not found in the low-drinking animals. High-drinking rats had lower risk-taking behavior prior to alcohol access and lower anxiety-like behavior after voluntary alcohol intake compared to low-drinking rats. In conclusion, the modified intermittent access paradigm may be useful for pharmacological manipulation of alcohol intake. With regard to behavior, the present findings highlights the importance of studying subgroup-dependent differences and add to the complexity of individual differences in behavioral traits of relevance to the vulnerability for excessive alcohol intake.

Place, publisher, year, edition, pages
2014. Vol. 275, 288-296 p.
Keyword [en]
Anxiety, Alcohol, Multivariate concentric square field (MCSF), Intermittent access, Individual difference, Alcohol deprivation effect
National Category
URN: urn:nbn:se:uu:diva-238391DOI: 10.1016/j.bbr.2014.08.058ISI: 000344431100037PubMedID: 25200519OAI: oai:DiVA.org:uu-238391DiVA: diva2:774331
Available from: 2014-12-22 Created: 2014-12-12 Last updated: 2015-11-13Bibliographically approved
In thesis
1. Individual differences in behavior, neurochemistry and pharmacology associated with voluntary alcohol intake
Open this publication in new window or tab >>Individual differences in behavior, neurochemistry and pharmacology associated with voluntary alcohol intake
2015 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Alcohol use disorder is a worldwide public health problem and is a disorder with substantial individual variation. There are suggested links between various behavioral traits, comorbid psychiatric diseases and excessive alcohol consumption. Moreover, the endogenous opioid system is involved in alcohol reward and reinforcement, and implicated in the action of alcohol. However, less is known about the complex associations between individual differences in behavior, alcohol consumption, pharmacotherapy response and related neurochemical mechanisms. Experimental animal models are critical for understanding the neurobiological underpinnings of alcohol use disorder.

The overall aims of this thesis were: i) to study the association between behavior and voluntary alcohol intake in outbred rats; ii) to study the association of voluntary alcohol intake, behavior, opioid receptor density and response to naltrexone; and iii) to obtain detailed behavioral characterizations of the animals on the basis of their voluntary alcohol intake.

The results revealed that the multivariate concentric square fieldTM (MCSF) test was a complementary method for understanding mechanisms underlying various mental states. The MCSF broadened the perspective on risk-related behaviors, including aspects of risk assessment. Individual differences in alcohol intake using the modified intermittent access paradigm enabled analyses of drinking patterns in high and low alcohol-drinking rats. There was an alcohol deprivation effect in high-drinking animals only. The behavior profiling of high alcohol drinking- rats before and after alcohol access suggested that this subgroup was consuming alcohol for its anxiolytic properties. Long-lasting changes were found in the mu and the delta opioid receptors after long-term, intermittent voluntary alcohol intake; some of these changes are in line with findings in humans. The voluntary alcohol consumption and the concomitant response to naltrexone were different for Wistar rats from different suppliers. Moreover, the Rcc Wistar rats may be more suitable for studies of alcohol use disorders due to increasing alcohol intake and the presence of a high-drinking subpopulation with increasing alcohol intake over time. The high-drinking subpopulation showed pronounced effects of naltrexone on alcohol intake.

In conclusion, studies of individual differences increase understanding of variability in behavior, pharmacotherapy response and factors involved in vulnerability of alcohol use disorders.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2015. 76 p.
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Pharmacy, ISSN 1651-6192 ; 205
intermittent access, multivariate concentric square field, open field, risk assessment, risk taking, individual difference, behavior, strain variation, endogenous opioid system, naltrexone
National Category
Basic Medicine
Research subject
Pharmaceutical Science
urn:nbn:se:uu:diva-264584 (URN)978-91-554-9378-3 (ISBN)
Public defence
2015-12-04, B21, BMC, Husargatan 3, Uppsala, 09:15 (English)
Available from: 2015-11-12 Created: 2015-10-15 Last updated: 2015-11-13

Open Access in DiVA

fulltext(783 kB)76 downloads
File information
File name FULLTEXT01.pdfFile size 783 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Momeni, ShimaRoman, Erika
By organisation
Department of Pharmaceutical Biosciences
In the same journal
Behavioural Brain Research

Search outside of DiVA

GoogleGoogle Scholar
Total: 76 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 369 hits
ReferencesLink to record
Permanent link

Direct link