Sensitive detection of aggregated prion protein via proximity ligation
2014 (English)In: Prion, ISSN 1933-6896, E-ISSN 1933-690X, Vol. 8, no 3, 261-265 p.Article in journal (Refereed) Published
The DNA assisted solid-phase proximity ligation assay (SP-PLA) provides a unique opportunity to specifically detect prion protein (PrP) aggregates by investigating the collocation of three or more copies of the specific protein. We have developed a SP-PLA that can detect PrP aggregates in brain homogenates from infected hamsters even after a 10(7)-fold dilution. In contrast, brain homogenate from uninfected animals did not generate a detectable signal at hundred-fold higher concentration. Using either of the two monoclonal anti-PrP antibodies 3F4 and 6H4 we successfully detected low concentrations of aggregated PrP. The presented results provide a proof of concept that this method might be an interesting tool in the development of diagnostic approaches of prion diseases.
Place, publisher, year, edition, pages
2014. Vol. 8, no 3, 261-265 p.
IdentifiersURN: urn:nbn:se:uu:diva-239529DOI: 10.4161/pri.32231ISI: 000348375500004PubMedID: 25482604OAI: oai:DiVA.org:uu-239529DiVA: diva2:774785