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Automated segmentation of free-lying cell nuclei in Pap smears for malignancy-associated change analysis
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2012 (English)In: Engineering in Medicine and Biology Society (EMBC), 2012 Annual International Conference of the IEEE, 2012, 5372-5375 p.Conference paper, Published paper (Refereed)
Abstract [en]

This paper presents an automated algorithm for robustly detecting and segmenting free-lying cell nuclei in bright-field microscope images of Pap smears. This is an essential initial step in the development of an automated screening system for cervical cancer based on malignancy associated change (MAC) analysis. The proposed segmentation algorithm makes use of gray-scale annular closings to identify free-lying nuclei-like objects together with marker-based watershed segmentation to accurately delineate the nuclear boundaries. The algorithm also employs artifact rejection based on size, shape, and granularity to ensure only the nuclei of intermediate squamous epithelial cells are retained. An evaluation of the performance of the algorithm relative to expert manual segmentation of 33 fields-of-view from 11 Pap smear slides is also presented. The results show that the sensitivity and specificity of nucleus detection is 94.71% and 85.30% respectively, and that the accuracy of segmentation, measured using the Dice coefficient, of the detected nuclei is 97.30 #x00B1;1.3%.

Place, publisher, year, edition, pages
2012. 5372-5375 p.
Keyword [en]
Dice coefficient;artifact rejection;automated algorithm;automated segmentation;bright field microscope;cervical cancer;free-lying cell nuclei;gray scale annular closing;intermediate squamous epithelial cells;malignancy associated change analysis;marker-based watershed segmentation;nuclear boundaries;pap smear;biomedical optical imaging;cancer;cellular biophysics;image segmentation;medical image processing;obstetrics;optical microscopy;
National Category
Medical Image Processing
Research subject
Computerized Image Analysis
Identifiers
URN: urn:nbn:se:uu:diva-188376DOI: 10.1109/EMBC.2012.6347208OAI: oai:DiVA.org:uu-188376DiVA: diva2:774830
Conference
IEEE EMBC 2012
Available from: 2014-12-29 Created: 2012-12-17 Last updated: 2014-12-29

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