Direct targeted quantitative molecular imaging of neurotransmitters in brain tissue sections
2014 (English)In: Neuron, ISSN 0896-6273, E-ISSN 1097-4199, Vol. 84, no 4, 697-707 p.Article in journal (Refereed) Published
Current neuroimaging techniques have very limited abilities to directly identify and quantify neurotransmitters from brain sections. We have developed a molecular-specific approach for the simultaneous imaging and quantitation of multiple neurotransmitters, precursors, and metabolites, such as tyrosine, tryptamine, tyramine, phenethylamine, dopamine, 3-methoxytyramine, serotonin, GABA, glutamate, acetylcholine, and L-alpha-glycerylphosphorylcholine, in histological tissue sections at high spatial resolutions. The method is employed to directly measure changes in the absolute and relative levels ofneurotransmitters in specific brain structures in animal disease models and in response to drug treatments, demonstrating the power of mass spectrometry imaging in neuroscience.
Place, publisher, year, edition, pages
2014. Vol. 84, no 4, 697-707 p.
IdentifiersURN: urn:nbn:se:uu:diva-239353DOI: 10.1016/j.neuron.2014.10.011ISI: 000345424900007PubMedID: 25453841OAI: oai:DiVA.org:uu-239353DiVA: diva2:774857