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Gene expression changes during Giardia-host cell interactions in serum-free medium
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
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2014 (English)In: Molecular and biochemical parasitology (Print), ISSN 0166-6851, E-ISSN 1872-9428, Vol. 197, no 1-2, 21-23 p.Article in journal (Refereed) Published
Abstract [en]

Serial Analysis of Gene Expression (SAGE) was used to quantify transcriptional changes in Giardia intestinalis during its interaction with human intestinal epithelial cells (IECs, HT-29) in serum free M199 medium. Transcriptional changes were compared to those in trophozoites alone in M199 and in TYI-S-33 Giardia growth medium. In total, 90 genes were differentially expressed, mainly those involved in cellular redox homeostasis, metabolism and small molecule transport but also cysteine proteases and structural proteins of the giardin family. Only 29 genes changed their expression due to IEC interaction and the rest were due to M199 medium. Although our findings generated a small dataset, it was consistent with our earlier microarray studies performed under different interaction conditions. This study has confined the number of genes in Giardia to a small subset that specifically change their expression due to interaction with IECs.

Place, publisher, year, edition, pages
2014. Vol. 197, no 1-2, 21-23 p.
National Category
Microbiology in the medical area
URN: urn:nbn:se:uu:diva-239591DOI: 10.1016/j.molbiopara.2014.09.007ISI: 000347129000004PubMedID: 25286381OAI: oai:DiVA.org:uu-239591DiVA: diva2:775085
Available from: 2014-12-30 Created: 2014-12-29 Last updated: 2015-02-04Bibliographically approved

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Svärd, Staffan
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