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Repetitive Measurements of Intrarenal Oxygenation In Vivo Using L Band Electron Paramagnetic Resonance
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology, Integrative Physiology.
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2014 (English)In: Advances in Experimental Medicine and Biology, ISSN 0065-2598, E-ISSN 2214-8019, Vol. 812, 135-141 p.Article in journal (Refereed) Published
Abstract [en]

Intrarenal oxygenation is heterogeneous with oxygen levels normally being highest in the superficial cortex and lowest in the inner medulla. Reduced intrarenal oxygenation has been implied in the pathology of several kidney diseases. However, there is currently no method available to repetitively monitor regional renal oxygenation using minimally invasive procedures. We therefore evaluated implantable lithium phthalocyanine (LiPc) probes, which display a close correlation between EPR line width and oxygen availability. LiPc probes were implanted in the kidney cortex and medulla in the same mouse and sEPR spectra were acquired using a L band scanner during inhalation of air (21 % oxygen) or a mixture of air and nitrogen (10 % oxygen). In order to separate the signals from the two probes, a 1 G/cm gradient was applied and the signals were derived from 40 consecutive sweeps. Peak-to-peak comparison of the EPR line was used to convert the signal to an approximate oxygen tension in MATLAB. Kidney cortex as well as medullary oxygenation was stable over the 45 day period (cortex 56 +/- 7 mmHg and medulla 43 +/- 6 mmHg). However, 10 % oxygen inhalation significantly reduced oxygenation in both cortex (56 +/- 6 to 34 +/- 2 mmHg n = 15 p < 0.05) and medulla (42 +/- 5 to 29 +/- 3 mmHg n = 7 p < 0.05). In conclusion, L band EPR using LiPc probes implanted in discrete intrarenal structures can be used to repetitively monitor regional renal oxygenation. This minimally invasive method is especially well suited for conditions of reduced intrarenal oxygenation since this increases the signal intensity which facilitates the quantification of the EPR signal to absolute oxygenation values.

Place, publisher, year, edition, pages
2014. Vol. 812, 135-141 p.
Keyword [en]
Kidney, LiPc, L-Band EPR, NMRI mice, Oxygenation
National Category
Physiology
Research subject
Physiology
Identifiers
URN: urn:nbn:se:uu:diva-240033DOI: 10.1007/978-1-4939-0620-8_18ISI: 000345121200019PubMedID: 24729225ISBN: 978-1-4939-0620-8 (print)ISBN: 978-1-4939-0583-6 (print)OAI: oai:DiVA.org:uu-240033DiVA: diva2:775807
Conference
41st Annual Meeting of the International-Society-on-Oxygen-Transport-to-Tissue (ISOTT), JUN 22-26, 2013, Hanover, NH
Available from: 2015-01-05 Created: 2015-01-05 Last updated: 2017-12-05

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Palm, Fredrik

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