Preparation and in vitro evaluation of strontium-doped calcium silicate/gypsum bioactive bone cement
2014 (English)In: Biomedical Materials, ISSN 1748-6041, E-ISSN 1748-605X, Vol. 9, no 4, 045002- p.Article in journal (Refereed) Published
The combination of two or more bioactive components with different biodegradability could cooperatively improve the physicochemical and biological performances of the biomaterials. Here we explore the use of alpha-calcium sulfate hemihydrate (alpha-CSH) and calcium silicate with and without strontium doping (Sr-CSi, CSi) to fabricate new bioactive cements with appropriate biodegradability as bone implants. The cements were fabricated by adding different amounts (0-35 wt%) of Sr-CSi (or CSi) into the alpha-CSH-based pastes at a liquid-to-solid ratio of 0.4. The addition of Sr-CSi into alpha-CSH cements not only led to a pH rise in the immersion medium, but also changed the surface reactivity of cements, making them more bioactive and therefore promoting apatite mineralization in simulated body fluid (SBF). The impact of additives on long-term in vitro degradation was evaluated by soaking the cements in Tris buffer, SBF, and alpha-minimal essential medium (alpha-MEM) for a period of five weeks. An addition of 20% Sr-CSi to alpha-CSH cement retarded the weight loss of the samples to 36% (in Tris buffer), 43% (in SBF) and 54% (in alpha-MEM) as compared with the pure alpha-CSH cement. However, the addition of CSi resulted in a slightly faster degradation in comparison with Sr-CSi in these media. Finally, the in vitro cell-ion dissolution products interaction study using human fetal osteoblast cells demonstrated that the addition of Sr-CSi improved cell viability and proliferation. These results indicate that tailorable bioactivity and biodegradation behavior can be achieved in gypsum cement by adding Sr-CSi, and such biocements will be of benefit for enhancing bone defect repair.
Place, publisher, year, edition, pages
2014. Vol. 9, no 4, 045002- p.
strontium-substituted calcium silicate, gypsum, bioactivity, biodegradability, composite cements
IdentifiersURN: urn:nbn:se:uu:diva-240161DOI: 10.1088/1748-6041/9/4/045002ISI: 000345614900002OAI: oai:DiVA.org:uu-240161DiVA: diva2:776581