Effects on neonatal exposure to the flame retardant tetrabrombisphenol-A, aluminum diethylphosphinate or zinc stannate on long-term, potentiation and synaptic protein levels in mice
2014 (English)In: Archives of Toxicology, ISSN 0340-5761, E-ISSN 1432-0738, Vol. 89, no 12, 2345-2354 p.Article in journal (Refereed) Published
Brominated flame retardants such as tetrabromobisphenol-A (TBBPA) may exert (developmental) neurotoxic effects. However, data on (neuro)toxicity of halogen-free flame retardants (HFFRs) are scarce. Recent in vitro studies indicated a high neurotoxic potential for some HFFRs, e.g., zinc stannate (ZS), whereas the neurotoxic potential of other HFFRs, such as aluminum diethylphosphinate (Alpi), appears low. However, the in vivo (neuro)toxicity of these compounds is largely unknown. We therefore investigated effects of neonatal exposure to TBBPA, Alpi or ZS on synaptic plasticity in mouse hippocampus. Male C57bl/6 mice received a single oral dose of 211 µmol/kg bw TBBPA, Alpi or ZS on postnatal day (PND) 10. On PND 17–19, effects on hippocampal synaptic plasticity were investigated using ex vivo extracellular field recordings. Additionally, we measured levels of postsynaptic proteins involved in long-term potentiation (LTP) as well as flame retardant concentrations in brain, muscle and liver tissues. All three flame retardants induced minor, but insignificant, effects on LTP. Additionally, TBBPA induced a minor decrease in post-tetanic potentiation. Despite these minor effects, expression of selected synaptic proteins involved in LTP was not affected. The flame retardants could not be measured in significant amounts in the brains, suggesting low bioavailability and/or rapid elimination/metabolism. We therefore conclude that a single neonatal exposure on PND 10 to TBBPA, Alpi or ZS does affect neurodevelopment and synaptic plasticity only to a small extent in mice. Additional data, in particular on persistence, bioaccumulation and (in vivo) toxicity, following prolonged (developmental) exposure are required for further (human) risk assessment.
Place, publisher, year, edition, pages
2014. Vol. 89, no 12, 2345-2354 p.
Flame retardants; Developmental neurotoxicity; Hippocampal synaptic plasticity; Tetrabromobisphenol-A (TBBPA); Aluminum diethylphosphinate (Alpi); Zinc stannate (ZS)
Other Biological Topics
Research subject Biology with specialization in Environmental Toxicology
IdentifiersURN: urn:nbn:se:uu:diva-240598DOI: 10.1007/s00204-014-1366-8ISI: 000366155200013PubMedID: 25253649OAI: oai:DiVA.org:uu-240598DiVA: diva2:776786
FunderEU, FP7, Seventh Framework Programme, FP7-ENV-2008-1-226563
De två första författarna har bidragit i lika hög grad2015-01-082015-01-082016-10-20Bibliographically approved