Human achaete-scute homologue 1 (HASH-1) is downregulated in differentiating neuroblastoma cells
1999 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 256, no 3, 557-563 p.Article in journal (Refereed) Published
The mammalian achaete-scute homologue, MASH-1, is crucial for early development of the sympathetic nervous system and is transiently expressed in sympathetic neuroblasts during embryogenesis. Here we report that the human homologue (HASH-1) was expressed in all analyzed cell lines (6/6) derived from the sympathetic nervous system tumor neuroblastoma. The majority of small-cell lung carcinoma (4/5) cell lines tested expressed HASH-1, while other nonneuronal/non-neuroendocrine cell lines were negative. Induced differentiation of neuroblastoma cells resulted in HASH-1 downregulation. This occurred concomitant with induction of neurite outgrowth and expression of the neuronal marker genes GAP-43 and neuropeptide Y. Constitutive expression of exogenous HASH-1 did not alter the capacity of the neuroblastoma cells to differentiate in response to differentiation-inducing agents. It is concluded that moderate HASH-1 expression does not compromise the capacity of these cells to differentiate.
Place, publisher, year, edition, pages
1999. Vol. 256, no 3, 557-563 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-241982DOI: 10.1006/bbrc.1999.0314PubMedID: 10080936OAI: oai:DiVA.org:uu-241982DiVA: diva2:781960