uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Brucellosis outbreak in a Swedish kennel in 2013: Determination of genetic markers for source tracing
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Microbiology and Infectious Medicine, Clinical Bacteriology. Swedish Forum for Biopreparedness Diagnostics, National Veterinary Institute of Sweden.
National Veterinary Institute of Sweden.
National Veterinary Institute of Sweden, Swedish Forum for Biopreparedness Diagnostics.
National Veterinary Institute of Sweden.
Show others and affiliations
2014 (English)In: Veterinary Microbiology, ISSN 0378-1135, E-ISSN 1873-2542, Vol. 174, no 3–4, 523-530 p.Article in journal (Refereed) Published
Abstract [en]

Brucellosis is a highly infectious zoonotic disease but rare in Sweden. Nonetheless, an outbreak of canine brucellosis caused by an infected dog imported to Sweden was verified in 2013. In total 25 dogs were tested at least duplicated by the following approaches: real-time PCR for the detection of Brucella canis, a Brucella genus-specific real-time PCR, selective cultivation, and microscopic examination. The whole genome of B. canis strain SVA13 was analysed regarding genetic markers for epidemiological examination. The genome of an intact prophage of Roseobacter was detected in B. canis strain SVA13 with whole genome sequence prophage analysis (WGS-PA). It was shown that the prophage gene content in the American, African and European isolates differs remarkably from the Asian strains. The prophage sequences in Brucella may therefore serve of use as genetic markers in epidemiological investigations. Phage DNA fragments were also detected in clustered, regularly interspaced short palindromic repeats (CRISPR) in the genome of strain SVA13. In addition to the recommendations for genetic markers in Brucella outbreak tracing, our paper reports a validated two-step stand-alone real-time PCR for the detection of B. canis and its first successful use in an outbreak investigation.

Place, publisher, year, edition, pages
2014. Vol. 174, no 3–4, 523-530 p.
Keyword [en]
Zoonosis, Brucellosis, Brucella canis, Whole genome sequencing phage analysis (WGS-PA), Clustered-regularly-interspaced short palindromic repeats (CRISPR), Source tracing
National Category
Research subject
Biology with specialization in Microbiology; Biology with specialization in Molecular Biology; Infectious Diseases; Forensic Medicine
URN: urn:nbn:se:uu:diva-242232DOI: 10.1016/j.vetmic.2014.10.015ISI: 000347601900029PubMedID: 25465667OAI: oai:DiVA.org:uu-242232DiVA: diva2:782873
Available from: 2015-01-22 Created: 2015-01-22 Last updated: 2015-03-06Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Kaden, Rene
By organisation
Clinical Bacteriology
In the same journal
Veterinary Microbiology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 208 hits
ReferencesLink to record
Permanent link

Direct link