Antigen Conjugated to Anti-CD23 Antibodies is Rapidly Transported to Splenic Follicles by Recirculating B Cells
2015 (English)In: Scandinavian Journal of Immunology, ISSN 0300-9475, E-ISSN 1365-3083, Vol. 81, no 1, 39-45 p.Article in journal (Refereed) Published
IgE-antigen complexes, administered intravenously to mice, induce a several 100-fold higher specific antibody response than antigen alone. Additionally, in vivo activation and proliferation of specific CD4(+) T cells is enhanced. The mechanism behind these effects is thought to be that peripheral B cells capture IgE-antigen complexes via their low-affinity receptor for IgE, CD23, and rapidly transport them to splenic B cell follicles where an immune response is initiated. Here, we demonstrate that ovalbumin, covalently coupled to anti-CD23 antibodies and administered intravenously to mice, is also transported to splenic follicles and induces an enhanced primary antibody response. These effects are absent in CD23-deficient mice. No enhanced induction of immunological memory was observed. These findings extend previous observations regarding the in vivo role of CD23 and emphasize that recirculating B cells play an important role in antigen transport to the spleen.
Place, publisher, year, edition, pages
2015. Vol. 81, no 1, 39-45 p.
Immunology in the medical area
IdentifiersURN: urn:nbn:se:uu:diva-242386DOI: 10.1111/sji.12248ISI: 000346771300006OAI: oai:DiVA.org:uu-242386DiVA: diva2:783576