Direct analysis of artemisinin in plasma and saliva using coupled-column high-performance liquid chromatography with a restricted-access material pre-column
2000 (English)In: Journal of Chromatography B: Biomedical Sciences and Applications, ISSN 1387-2273, Vol. 742, no 1, 155-162 p.Article in journal (Refereed) Published
A previously established HPLC system with post-column derivatization for the analysis of artemisinin was coupled to an ADS (alkyl-diol silica) pre-column, allowing direct and repetitive injection of protein-rich fluids such as plasma. The limit of quantitation for 100 μl of plasma was 10 ng/ml (CV=10.5%) while concentrations down to 2 ng/ml could be quantified for 1.00 ml saliva samples (CV=11.1%). The system was linear in the tested range of 10–2000 ng/ml for plasma and 2–240 ng/ml for saliva samples, respectively. This paper introduces coupled column HPLC as a simplified method for the routine analysis of artemisinin in biological fluids.
Place, publisher, year, edition, pages
2000. Vol. 742, no 1, 155-162 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-50604DOI: 10.1016/S0378-4347(00)00156-0OAI: oai:DiVA.org:uu-50604DiVA: diva2:78512