Metabolic profiling of epithelial ovarian cancer cell lines: evaluation of harvesting protocols for profiling using NMR spectroscopy
2015 (English)In: Bioanalysis, ISSN 1757-6180, E-ISSN 1757-6199, Vol. 7, no 2, 157-166 p.Article in journal (Refereed) Published
BACKGROUND: Metabolic profiling represents a novel technology for analyzing tumor cells. Epithelial ovarian carcinoma has a low survival rate due to the development of aggressive and chemotherapy-resistant cells. A tailored and reliable protocol is presented for profiling of chemoresistant cells using the cell line SKOV3 and a multiresistant subline SKOV3R.
RESULTS: Harvesting protocols with cold methanol or MilliQ freeze/thaw cycles were compared. Increased reproducibility using MilliQ was evidenced. Importantly, both approaches resulted in similar profiles. Compared with parental SKOV3, the SKOV3R cells showed a significantly different profile.
CONCLUSION: The MilliQ protocol is preferred owing to higher reproducibility and increased sample preparation options. The resulting metabolic profiles summarize metabolic alterations in chemoresistant cells consistent with a progressed and aggressive phenotype.
Place, publisher, year, edition, pages
2015. Vol. 7, no 2, 157-166 p.
Analytical Chemistry Pharmaceutical Sciences
IdentifiersURN: urn:nbn:se:uu:diva-243094DOI: 10.4155/bio.14.235ISI: 000354284100003PubMedID: 25587833OAI: oai:DiVA.org:uu-243094DiVA: diva2:785969
De två första författarna delar förstaförfattarskapet.2015-02-042015-02-042015-06-22Bibliographically approved