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Metabolic profiling of epithelial ovarian cancer cell lines: evaluation of harvesting protocols for profiling using NMR spectroscopy
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Analytical Pharmaceutical Chemistry.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Analytical Pharmaceutical Chemistry.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Analytical Pharmaceutical Chemistry.
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2015 (English)In: Bioanalysis, ISSN 1757-6180, E-ISSN 1757-6199, Vol. 7, no 2, 157-166 p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Metabolic profiling represents a novel technology for analyzing tumor cells. Epithelial ovarian carcinoma has a low survival rate due to the development of aggressive and chemotherapy-resistant cells. A tailored and reliable protocol is presented for profiling of chemoresistant cells using the cell line SKOV3 and a multiresistant subline SKOV3R.

RESULTS: Harvesting protocols with cold methanol or MilliQ freeze/thaw cycles were compared. Increased reproducibility using MilliQ was evidenced. Importantly, both approaches resulted in similar profiles. Compared with parental SKOV3, the SKOV3R cells showed a significantly different profile.

CONCLUSION: The MilliQ protocol is preferred owing to higher reproducibility and increased sample preparation options. The resulting metabolic profiles summarize metabolic alterations in chemoresistant cells consistent with a progressed and aggressive phenotype.

Place, publisher, year, edition, pages
2015. Vol. 7, no 2, 157-166 p.
National Category
Analytical Chemistry Pharmaceutical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-243094DOI: 10.4155/bio.14.235ISI: 000354284100003PubMedID: 25587833OAI: oai:DiVA.org:uu-243094DiVA: diva2:785969
Note

De två första författarna delar förstaförfattarskapet.

Available from: 2015-02-04 Created: 2015-02-04 Last updated: 2017-12-05Bibliographically approved

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Engskog, Mikael K RHaglöf, JakobArvidsson, TorbjörnPettersson, Curt

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