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Opioid effects on 45Ca2+ uptake and glutamate release in rat cerebral cortex in primary culture
Department of Clinical Neuroscience, Experimental Alcohol and Drug Addiction Research Section, Karolinska Institute. (Pharmacology)
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1997 (English)In: Journal of Neurochemistry, ISSN 0022-3042, E-ISSN 1471-4159, Vol. 68, no 2, 517-524 p.Article in journal (Refereed) Published
Abstract [en]

Primary cultures of rat cortex, conveniently prepared from newborn animals, were used to study opioid effects on 45Ca2+ uptake and glutamate release. 45Ca2+ uptake, induced by treatment with glutamate or NMDA, was largely blocked by the NMDA antagonist MK-801. K+ depolarization-induced 45Ca2+ uptake was also reduced by MK-801, indicating that the effect was mediated by glutamate release. Direct analysis verified that glutamate, and aspartate, were indeed released. Opioid peptides of the prodynorphin system were also released and these, or other peptides, were functionally active, because naloxone treatment increased glutamate release, as well as the 45Ca2+ uptake induced by depolarization. Opioid agonists, selective for mu-, kappa-, and delta-receptors, inhibited the 45Ca2+ uptake induced by K+ depolarization. The combination of low concentrations of MK-801 and opioid agonists resulted in additive inhibition of K(+)-induced 45Ca2+ uptake. The results indicate that this system may be useful as an in vitro CNS model for studying modulation by opioids of glutamate release and Ca2+ uptake under acute, and perhaps also chronic, opiate treatment.

Place, publisher, year, edition, pages
1997. Vol. 68, no 2, 517-524 p.
Keyword [en]
primary culture, rat cerebral cortex, dynorphin peptides, glutamate release, Ca-45(2+) uptake, opiate agonists, naloxone
National Category
Medical and Health Sciences Pharmaceutical Sciences
URN: urn:nbn:se:uu:diva-50726DOI: 10.1046/j.1471-4159.1997.68020517.xISI: A1997WD07900008PubMedID: 9003036OAI: oai:DiVA.org:uu-50726DiVA: diva2:78635
Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2011-03-10Bibliographically approved

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