uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
A comparison of microwave irradiation and decapitation. Basal levels of dynorphin and enkephalin peptides and the effect of morphine treatment on dynorphin peptides
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences. (Pharmacology)
Show others and affiliations
1997 (English)In: Neuropeptides, ISSN 0143-4179, E-ISSN 1532-2785, Vol. 31, no 4, 357-365 p.Article in journal (Refereed) Published
Abstract [en]

Opioid peptides were analysed in tissue extracts of various brain structures and the pituitary gland from rats sacrificed by microwave irradiation, and compared with peptide levels in tissue extracts from decapitated rats. Dynorphin A, dynorphin B and Leu-enkephalinArg6, derived from prodynorphin, and Met-enkephalinArg6Phe7 from proenkephalin, were measured. Basal immunoreactive levels of dynorphin A and B were consistently higher in extracts from microwave-irradiated rats, whereas in these extracts immunoreactive levels of Leu-enkephalinArg6, an endogenous metabolite of dynorphin peptides, were either lower than, the same as or higher than in decapitated rats. Immunoreactive levels of Met-enkephalinArg6Phe7 were higher in microwave-irradiated rats. Effects of morphine treatment on prodynorphin peptide levels were evaluated and compared with previous findings in decapitated rats. Dynorphin immunoreactive levels were higher in the nucleus accumbens and striatum of morphine-tolerant rats than in corresponding areas in saline-treated rats. These results indicate tissue-specific metabolism of prodynorphin peptides and show that metabolism of opioid peptides occurs during the dissection procedure after decapitation of the rat even though precautions are taken to minimize degradation.

Place, publisher, year, edition, pages
1997. Vol. 31, no 4, 357-365 p.
National Category
Medical and Health Sciences Pharmaceutical Sciences
URN: urn:nbn:se:uu:diva-50728DOI: 10.1016/S0143-4179(97)90072-XISI: A1997XX36700011PubMedID: 9308024OAI: oai:DiVA.org:uu-50728DiVA: diva2:78637
Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2011-03-08Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Nylander, Ingrid
By organisation
Department of Pharmaceutical Biosciences
In the same journal
Medical and Health SciencesPharmaceutical Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 110 hits
ReferencesLink to record
Permanent link

Direct link