uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Immune response and functional role of antibodies raised in heifers against a Staphylococcus aureus CP5 lysate and recombinant antigens vaccine formulated with Iscom Matrix adjuvant
Show others and affiliations
2014 (English)In: Veterinary Immunology and Immunopathology, ISSN 0165-2427, E-ISSN 1873-2534, Vol. 162, no 3-4, 96-107 p.Article in journal (Refereed) Published
Abstract [en]

Staphylococcus aureus is the most frequently isolated pathogen from bovine intramammary infections worldwide. Commercially available vaccines for mastitis control are composed either of S. aureus lysates or inactivated whole-cells formulated with traditional adjuvants. We recently showed the ability of a S. aureus CP5 lysate vaccine adjuvanted with Iscom Matrix to generate a longer lasting specific antibody response in blood and milk with improved opsonic capacity compared with a S. aureus CP5 whole-cell formulation. The aim of the present study was to obtain an experimental immunogen composed of lysed cells of a CP5 S. aureus strain supplemented with recombinant clumping factor A fibronectin binding protein A and beta-toxin formulated with Iscom Matrix characterize the immune response generated when immunizing pregnant heifers and assess the functional role of antibodies raised against this immunogen in experimental models. Both a lysate vaccine and a lysate + recombinant antigens vaccine elicited antibodies that promoted neutrophil phagocytosis and inhibited internalization into mammary epithelial cells in vitro. Incorporation of defined antigenic molecules to the lysate formulation elicited a strong specific humoral immune response against both lysate and recombinant antigens and was associated with higher expression of regulatory and pro-inflammatory cytokines. In addition antibodies were efficient for blocking S. aureus binding to bovine fibrinogen and fibronectin and neutralizing beta-toxin effect in vitro placing these antigens as candidates to be included in a formulation directed to prevent staphylococcal bovine mastitis. (C) 2014 Elsevier B.V. All rights reserved.

Place, publisher, year, edition, pages
2014. Vol. 162, no 3-4, 96-107 p.
Keyword [en]
Staphylococcus aureus, Bovine mastitis, Immunization, Lysate, Recombinant antigens, Iscom Matrix
National Category
Immunology in the medical area
URN: urn:nbn:se:uu:diva-244517DOI: 10.1016/j.vetimm.2014.10.004ISI: 000347767800004OAI: oai:DiVA.org:uu-244517DiVA: diva2:788951
Available from: 2015-02-17 Created: 2015-02-17 Last updated: 2015-02-17Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text
By organisation
Clinical Virology
In the same journal
Veterinary Immunology and Immunopathology
Immunology in the medical area

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 1037 hits
ReferencesLink to record
Permanent link

Direct link