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Extensive duplication of the Wolbachia DNA in chromosome four of Drosophila ananassae
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Evolution.
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2014 (English)In: BMC Genomics, ISSN 1471-2164, E-ISSN 1471-2164, Vol. 15, 1097- p.Article in journal (Refereed) Published
Abstract [en]

Background: Lateral gene transfer (LGT) from bacterial Wolbachia endosymbionts has been detected in similar to 20% of arthropod and nematode genome sequencing projects. Many of these transfers are large and contain a substantial part of the Wolbachia genome. Results: Here, we re-sequenced three D. ananassae genomes from Asia and the Pacific that contain large LGTs from Wolbachia. We find that multiple copies of the Wolbachia genome are transferred to the Drosophila nuclear genome in all three lines. In the D. ananassae line from Indonesia, the copies of Wolbachia DNA in the nuclear genome are nearly identical in size and sequence yielding an even coverage of mapped reads over the Wolbachia genome. In contrast, the D. ananassae lines from Hawaii and India show an uneven coverage of mapped reads over the Wolbachia genome suggesting that different parts of these LGTs are present in different copy numbers. In the Hawaii line, we find that this LGT is underrepresented in third instar larvae indicative of being heterochromatic. Fluorescence in situ hybridization of mitotic chromosomes confirms that the LGT in the Hawaii line is heterochromatic and represents similar to 20% of the sequence on chromosome 4 (dot chromosome, Muller element F). Conclusions: This collection of related lines contain large lateral gene transfers composed of multiple Wolbachia genomes that constitute >2% of the D. ananassae genome (similar to 5 Mbp) and partially explain the abnormally large size of chromosome 4 in D. ananassae.

Place, publisher, year, edition, pages
2014. Vol. 15, 1097- p.
Keyword [en]
Drosophila ananassae, Wolbachia, Lateral gene transfer, Horizontal gene transfer, Symbiosis, Underreplication, Heterochromatin
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-243684DOI: 10.1186/1471-2164-15-1097ISI: 000347575100001PubMedID: 25496002OAI: oai:DiVA.org:uu-243684DiVA: diva2:789408
Available from: 2015-02-18 Created: 2015-02-11 Last updated: 2017-12-04Bibliographically approved

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Klasson, LisaAndersson, Siv G. E.

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