uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Arabidopsis cytosolic acyl-CoA-binding proteins ACBP4, ACBP5 and ACBP6 have overlapping but distinct roles in seed development
Show others and affiliations
2014 (English)In: Bioscience Reports, ISSN 0144-8463, E-ISSN 1573-4935, Vol. 34, no 6, 865-877 p.Article in journal (Refereed) Published
Abstract [en]

Eukaryotic cytosolic ACBPs (acyl-CoA-binding proteins) bind acyl-CoA esters and maintain a cytosolic acyl-CoA pool, but the thermodynamics of their protein-lipid interactions and physiological relevance in plants are not well understood. Arabidopsis has three cytosolic ACBPs which have been identified as AtACBP4, AtACBP5 and AtACBP6, and microarray data indicated that all of them are expressed in seeds; AtACBP4 is expressed in early embryogenesis, whereas AtACBP5 is expressed later. ITC (isothermal titration calorimetry) in combination with transgenic Arabidopsis lines were used to investigate the roles of these three ACBPs from Arabidopsis thaliana. The dissociation constants, stoichiometry and enthalpy change of AtACBP interactions with various acyl-CoA esters were determined using ITC. Strong binding of recombinant (r) AtACBP6 with long-chain acyl-CoA (C16-to C18-CoA) esters was observed with dissociation constants in the nanomolar range. However, the affinity of rAtACBP4 and rAtACBP5 to these acyl-CoA esters was much weaker (dissociation constants in the micromolar range), suggesting that they interact with acyl-CoA esters differently from rAtACBP6. When transgenic Arabidopsis expressing AtACBP6pro::GUS was generated, strong GUS (beta-glucuronidase) expression in cotyledonary-staged embryos and seedlings prompted us to measure the acyl-CoA contents of the acbp6 mutant. This mutant accumulated higher levels of C18:1-CoA and C18:1- and C18:2-CoAs in cotyledonary-staged embryos and seedlings, respectively, in comparison with the wild type. The acbp4acbp5acbp6 mutant showed the lightest seed weight and highest sensitivity to abscisic acid during germination, suggesting their physiological functions in seeds.

Place, publisher, year, edition, pages
2014. Vol. 34, no 6, 865-877 p.
Keyword [en]
acyl-CoA-binding protein, isothermal titration calorimetry, lipid metabolism, seed
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:uu:diva-244610DOI: 10.1042/BSR20140139ISI: 000347799400019PubMedID: 25423293OAI: oai:DiVA.org:uu-244610DiVA: diva2:789618

Article Number: e00165

Available from: 2015-02-19 Created: 2015-02-18 Last updated: 2015-02-19Bibliographically approved

Open Access in DiVA

fulltext(1191 kB)53 downloads
File information
File name FULLTEXT01.pdfFile size 1191 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Sooriyaarachchi, SanjeewaniMowbray, Sherry L.
By organisation
Structure and Molecular BiologyScience for Life Laboratory, SciLifeLab
In the same journal
Bioscience Reports
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 53 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 188 hits
ReferencesLink to record
Permanent link

Direct link