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The Use of MALDI Imaging Mass Spectrometry for the Detection of Small Heat Shock Protein and Neuropeptides in Parkinson's Disease and Drug Addiction
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences. University of Bath.
2015 (English)Independent thesis Advanced level (professional degree), 20 HE creditsStudent thesis
Abstract [en]

Parkinson’s disease is a progressive neurodegenerative movement disorder, which affects 1-2% adults over 50 years of age. Levodopa is used in Parkinson’s disease treatment to control and reduce debilitating symptoms, however long-term levodopa treatment often leads to development of involuntary movements, known as levodopa-induced dyskinesias. AlphaB-crystallin (CRYAB) is a small heat shock protein that has been found to be up-regulated in the brains of Parkinsonian rats and those with a high level of dyskinesia following levodopa treatment. This study uses matrix-assisted laser desorption ionisation (MALDI) imaging mass spectrometry (IMS) to look further into the localisation of intact and digested CRYAB in healthy brain tissue, and intact CRYAB in brain tissue with unilateral 6- OHDA lesions, as this is a Parkinson’s disease model. Investigations into whether CRYAB can interact with other soluble factors are carried out and then analysed using MALDI IMS. Microdialysis samples from morphine-treated mice are analysed to determine whether CRYAB is secreted upon stimulation of the dopaminergic system, and other peptides secreted are also investigated. The investigations into CRYAB interacting with soluble factors and microdialysis samples from morphine-treated mice did not show clear results. The results from healthy brain tissue show that it is possible to detect intact and digested CRYAB using MALDI IMS. However, they did not give a clear indication of brain areas with prominent CRYAB levels. The structural investigations involving brain tissue with 6-OHDA lesions showed increased CRYAB levels in the corpus callosum in 2 out of 3 rats, with a further increase on the lesion side of the brain. These results are promising but warrant further investigation.

Place, publisher, year, edition, pages
2015. , 26 p.
National Category
Pharmacology and Toxicology
Identifiers
URN: urn:nbn:se:uu:diva-244861OAI: oai:DiVA.org:uu-244861DiVA: diva2:790018
Subject / course
Pharmaceutical Biosciences
Educational program
Master of Science Programme in Pharmacy
Supervisors
Examiners
Available from: 2017-05-11 Created: 2015-02-23 Last updated: 2017-05-11Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association
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Language
  • de-DE
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Output format
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