uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Imaging single cells in a beam of live cyanobacteria with an X-ray laser
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular biophysics.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular biophysics.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular biophysics.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular biophysics.
Show others and affiliations
2015 (English)In: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 6, 5704Article in journal (Refereed) Published
Abstract [en]

There exists a conspicuous gap of knowledge about the organization of life at mesoscopic levels. Ultra-fast coherent diffractive imaging with X-ray free-electron lasers can probe structures at the relevant length scales and may reach sub-nanometer resolution on micron-sized living cells. Here we show that we can introduce a beam of aerosolised cyanobacteria into the focus of the Linac Coherent Light Source and record diffraction patterns from individual living cells at very low noise levels and at high hit ratios. We obtain two-dimensional projection images directly from the diffraction patterns, and present the results as synthetic X-ray Nomarski images calculated from the complex-valued reconstructions. We further demonstrate that it is possible to record diffraction data to nanometer resolution on live cells with X-ray lasers. Extension to sub-nanometer resolution is within reach, although improvements in pulse parameters and X-ray area detectors will be necessary to unlock this potential.

Place, publisher, year, edition, pages
2015. Vol. 6, 5704
National Category
Structural Biology
Identifiers
URN: urn:nbn:se:uu:diva-245040DOI: 10.1038/ncomms6704ISI: 000350034400002PubMedID: 25669616OAI: oai:DiVA.org:uu-245040DiVA: diva2:790292
Available from: 2015-02-24 Created: 2015-02-24 Last updated: 2017-12-04Bibliographically approved
In thesis
1.
The record could not be found. The reason may be that the record is no longer available or you may have typed in a wrong id in the address field.

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Authority records BETA

van der Schot, GijsSvenda, MartinMaia, Filipe R. N. C.Hantke, MaxSeibert, M. MarvinAndreasson, JakobTimneanu, NicusorWestphal, DanielHasse, DirkCarlsson, Gunilla H.Larsson, Daniel S. D.Andersson, IngerHajdu, JanosEkeberg, Tomas

Search in DiVA

By author/editor
van der Schot, GijsSvenda, MartinMaia, Filipe R. N. C.Hantke, MaxSeibert, M. MarvinAndreasson, JakobTimneanu, NicusorWestphal, DanielHasse, DirkCarlsson, Gunilla H.Larsson, Daniel S. D.Andersson, IngerHajdu, JanosEkeberg, Tomas
By organisation
Molecular biophysicsMolecular and condensed matter physics
In the same journal
Nature Communications
Structural Biology

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 1121 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf