Zebrafish: A possible tool to evaluate bioactive ions
2015 (English)In: Acta Biomaterialia, ISSN 1742-7061, E-ISSN 1878-7568, Vol. 19, 10-14 p.Article in journal (Refereed) Published
Zebrafish is a well-established model organism with a skeletal structure that highly resembles mammalian bone. Yet its use in the research field of biomaterials has been limited. One area that could benefit from this model system is the evaluation of ionic dissolution products from different materials. As a proof of concept we have evaluated the effect of silicate ions on the zebrafish larvae and compared it to a well-known osteblastic cell line, MC3T3-E1 subclone 14. We have shown that sodium metasilicate (125 mu M and 625 mu M) induces more mineralisation in a dose-dependent manner in zebrafish larvae, 9 days post fertilisation as compared to the non-treated group. Moreover the same trends were seen when adding sodium metasilicate to MC3T3-E1 cultures, with more mineralisation and higher ALP levels with higher doses of silicate (25, 125 and 625 mu M). These results indicate the feasibility of zebrafish larvae for ionic dissolution studies. The zebrafish model is superior to isolated cell cultures in the aspect that it includes the whole bone remodelling system, with osteoblasts, osteoclasts and osteocytes. Zebrafish could thus provide a powerful in vivo tool and be a bridge between cell culture systems and mammalian models.
Place, publisher, year, edition, pages
2015. Vol. 19, 10-14 p.
Zebrafish, Silicon, MC3T3-E1, Bone mineralisation
IdentifiersURN: urn:nbn:se:uu:diva-245779DOI: 10.1016/j.actbio.2015.03.010ISI: 000354152100002PubMedID: 25770927OAI: oai:DiVA.org:uu-245779DiVA: diva2:792703
FunderSwedish Research Council, GA 621-2011-3399