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A General Method for Rapid Determination of Antibiotic Susceptibility and Species in Bacterial Infections
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
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2015 (English)In: Journal of Clinical Microbiology, ISSN 0095-1137, E-ISSN 1098-660X, Vol. 53, no 2, 425-432 p.Article in journal (Refereed) Published
Abstract [en]

To ensure correct antibiotic treatment and reduce the unnecessary use of antibiotics, there is an urgent need for new rapid methods for species identification and determination of antibiotic susceptibility in infectious pathogenic bacteria. We have developed a general method for the rapid identification of the bacterial species causing an infection and the determination of their antibiotic susceptibility profiles. An initial short cultivation step in the absence and presence of different antibiotics was combined with sensitive species-specific padlock probe detection of the bacterial target DNA to allow a determination of growth (i.e., resistance) and no growth (i.e., susceptibility). A proof-of-concept was established for urinary tract infections in which we applied the method to determine the antibiotic susceptibility profiles of Escherichia coli for two drugs with 100% accuracy in 3.5 h. The short assay time from sample to readout enables fast appropriate treatment with effective drugs and minimizes the need to prescribe broad-spectrum antibiotics due to unknown resistance profiles of the treated infection.

Place, publisher, year, edition, pages
2015. Vol. 53, no 2, 425-432 p.
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:uu:diva-246828DOI: 10.1128/JCM.02434-14ISI: 000348568500009PubMedID: 25411178OAI: oai:DiVA.org:uu-246828DiVA: diva2:794219
Note

De två sista författarna delar sistaförfattarskapet.

Available from: 2015-03-10 Created: 2015-03-10 Last updated: 2017-12-04Bibliographically approved

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Zorzet, AnnaAndersson, Dan I.

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