uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Differential regulation of CD40-mediated human B cell responses by antibodies directed against different CD40 epitopes
Show others and affiliations
2000 (English)In: Cellular Immunology, ISSN 0008-8749, E-ISSN 1090-2163, Vol. 201, no 2, 109-123 p.Article in journal (Refereed) Published
Abstract [en]

Ligation of CD40 using anti-CD40 or soluble CD40-ligand activates numerous intracellular kinases which transduce signals to the nucleus. The nature whereby these signaling events are coupled to distal functional events in B cells is poorly understood. In this study, using anti-CD40 monoclonal antibodies which recognize different epitopes on CD40, we compare the ability to activate the stress-activated protein kinases (SAPK) such as c-Jun NH(2) terminal kinase and p38 in human B cells with CD40 function. Activation of the SAPK pathway correlated with levels of activation of Rel/NF-kappaB transcription factors, but did not appear to be associated with rescue from anti-IgM induced apoptosis by suppressing caspase (CPP32) activity. Somewhat surprisingly, in the presence of IL-4, those antibodies to CD40 which failed to activate SAPK were most active in IgE production. IgE production was augmented in the presence of wortmannin. These studies suggest that rescue from apoptosis and IgE production mediated via CD40 may be independent of SAPK activation, induction of Rel/NF-kappaB, or suppression of CPP32 and that IgE production is, at least in part, regulated by signaling pathways that are dependent on phosphatidylinositol 3-kinase.

Place, publisher, year, edition, pages
2000. Vol. 201, no 2, 109-123 p.
Keyword [en]
CD40, cell signaling, B lymphocytes, MAP kinases
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-51577DOI: 10.1006/cimm.2000.1645PubMedID: 10831320OAI: oai:DiVA.org:uu-51577DiVA: diva2:79486
Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2011-06-28Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Gerwins, Pär
By organisation
Department of Genetics and PathologyDepartment of Medical Biochemistry and Microbiology
In the same journal
Cellular Immunology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 176 hits
ReferencesLink to record
Permanent link

Direct link