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Determination of the antimalarial drug piperaquine in small volume pediatric plasma samples by LC-MS/MS
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
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2014 (English)In: Bioanalysis, ISSN 1757-6180, E-ISSN 1757-6199, Vol. 6, no 23, 3081-3089 p.Article in journal (Refereed) Published
Abstract [en]

Aim: Determination of piperaquine (PQ) in pediatric plasma requires a method with a small sample volume. Results: We report a sensitive LC-MS/MS method for quantitation of PQ with only 25 mu l human plasma. Using a deuterated internal standard (PQ-d(6)), an analytical PFP column, APCI(+) as the ion source and MRM (535/288 for PQ and 541/294 for the IS) for detection, the method has a linear calibration range of 1.5-250 ng/ml with a runtime of 3.0 min per sample. The method was applied to plasma samples from children. Conclusion: The developed LC-MS/MS method is suitable for pediatric studies with small volume plasma samples collected via capillary tubes. One limitation was the performance of PFP columns varied among different brands.

Place, publisher, year, edition, pages
2014. Vol. 6, no 23, 3081-3089 p.
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Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-247353DOI: 10.4155/bio.14.254ISI: 000349186300004PubMedID: 25529877OAI: oai:DiVA.org:uu-247353DiVA: diva2:796891
Available from: 2015-03-20 Created: 2015-03-17 Last updated: 2017-12-04Bibliographically approved

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