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2 '-N-Guanidino, 4 '-C-ethylene bridged thymidine (GENA-T) modified oligonucleotide exhibits triplex formation with excellent enzymatic stability
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry.
2015 (English)In: RSC Advances, ISSN 2046-2069, E-ISSN 2046-2069, Vol. 5, no 16, 12257-12260 p.Article in journal (Refereed) Published
Abstract [en]

Here we present the synthesis and characterization of a new 2'-N-Guanidino, 4'-C-ethylene bridged thymidine (GENA-T) modified oligonucleotide possessing North-locked sugar conformation. Incorporation of GENA-T nucleotide though did not change the thermal stability of the oligonucleotides toward the complementary RNA; it significantly increased the stability of the parallel triplex at pH 7. The melting temperature of the triplex was increased by +9.5 degrees C as compared to that of the isosequential unmodified sequence. Moreover this modification imparted exceptional nuclease stability to the oligonucleotides for over 33 h. This study clearly demonstrates that GENA-T modified oligonucleotides could improve triplex formation with phenomenal enzymatic stability and could be used for various biomedical applications.

Place, publisher, year, edition, pages
2015. Vol. 5, no 16, 12257-12260 p.
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-247338DOI: 10.1039/c4ra14721aISI: 000348985400071OAI: oai:DiVA.org:uu-247338DiVA: diva2:797054
Available from: 2015-03-22 Created: 2015-03-17 Last updated: 2017-12-04Bibliographically approved

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Oommen, Oommen PodiyanVarghese, Oommen P.

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