Receptor-mediated Mn2+ influx in rat hepatocytes: comparison of cells loaded with Fura-2 ester and cells microinjected with Fura-2 salt
1994 (English)In: Biochemical Journal, ISSN 0264-6021, E-ISSN 1470-8728, Vol. 302, 5-9 p.Article in journal (Refereed) Published
In single Fura-2 ester-loaded hepatocytes, stimulation by vasopressin, but not emptying of the agonist-sensitive Ca2+ store by 2,5-di-(t-butyl)hydroquinone, resulted in an increase in the rate of Fura-2 fluorescence-quenching by Mn2+. Similarly, in cells microinjected with Fura-2 salt, vasopressin stimulated Mn2+ entry while 2,5-di-(t-butyl)hydroquinone or thapsigargin did not. The pattern of Fura-2 quenching by Mn2+ only correlated with the movement of Mn2+ across the plasma membrane.
Place, publisher, year, edition, pages
1994. Vol. 302, 5-9 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-248129ISI: A1994PC82800002PubMedID: 8068023OAI: oai:DiVA.org:uu-248129DiVA: diva2:798947