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Two separate plasma membrane Ca2+ carriers participate in receptor-mediated Ca2+ influx in rat hepatocytes.
Rolf Luft Center for Diabetes Research, Department of Endocrinology, Karolinska Institutet, Stockholm, Sweden.
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1994 (English)In: Biochimica et Biophysica Acta, ISSN 0006-3002, Vol. 1223, no 2, 226-33 p.Article in journal (Refereed) Published
Abstract [en]

The plasma membrane Ca2+ carrier system involved in receptor-mediated Ca2+ entry was studied. Using the Ca2+ readdition protocol, the rate of cytosolic free Ca2+ concentration ([Ca2+]i) increase in vasopressin-pretreated hepatocytes was significantly higher than in thapsigargin- or 2,5-di(tert-butyl)hydroquinone-pretreated cells. The addition of Mn2+ to unstimulated hepatocytes resulted in a biphasic quench of fura-2 fluorescence. After an initial phase that was fast in rate but of short duration, the rate of fura-2 quench by Mn2+ became much slower and lasted until all the cellular fura-2 was quenched. Pretreatment of the cells with vasopressin only accelerated the rate of the latter phase but not of the initial one. In agonist-stimulated cells, acidification of the extracellular medium or the presence of ruthenium red, econazole or SK&F 96365 decreased the rates of both [Ca2+]i increase and Mn2+ entry upon addition of the respective cation. By contrast, neomycin and N-tosyl-L-phenylalanine chloromethyl ketone markedly decreased the rate of [Ca2+]i increase upon Ca2+ readdition but had no effect on vasopressin-stimulated Mn2+ entry. None of the treatments affected the ability of vasopressin and thapsigargin to mobilize the internal Ca2+ store. It is concluded that in hepatocytes the two pathways of receptor-mediated Ca2+ entry control two distinct yet pharmacologically related cation carriers.

Place, publisher, year, edition, pages
1994. Vol. 1223, no 2, 226-33 p.
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Other Medical Sciences
URN: urn:nbn:se:uu:diva-248132DOI: 10.1016/0167-4889(94)90230-5ISI: A1994PG04000009PubMedID: 8086492OAI: oai:DiVA.org:uu-248132DiVA: diva2:799288
Available from: 2015-03-30 Created: 2015-03-27 Last updated: 2015-04-08Bibliographically approved

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