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Profiling sulfation/epimerization pattern of full-length heparan sulfate by NMR following cell culture C-13-glucose metabolic labeling
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
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2015 (English)In: Glycobiology, ISSN 0959-6658, E-ISSN 1460-2423, Vol. 25, no 2, 151-156 p.Article in journal (Refereed) Published
Abstract [en]

Through its ability to interact with proteins, heparan sulfate (HS) fulfills a large variety of functions. Protein binding depends on the level of HS sulfation and epimerization which are cell specific and dynamically regulated. Characterization of this molecule, however, has been restricted to oligosaccharide fragments available in large amount for structural investigation or to sulfate distribution through compositional analysis. Here we developed a H-1-C-13 2D NMR-based approach, directly performed on HS isolated from C-13-labeled cells. By integrating the peak volumes measured at different chemical shifts, this non-destructive analysis allows us to determine both the sulfation and the iduronic/glucuronic profiles of the polysaccharide. Applied to wild-type and N-deacetylase/N-sulfotransferase-deficient fibroblasts as well as to epithelial cells differentiation, it also gives insights into the functional relationships existing between HS biosynthetic enzymes. This approach should be of significant interest to better understand HS changes that occur through physiologic regulations or during pathological development.

Place, publisher, year, edition, pages
2015. Vol. 25, no 2, 151-156 p.
Keyword [en]
C-13-metabolic labeling, heparan sulfate, quantification of GlcA/IdoA epimeriation, sulfation pattern
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:uu:diva-248468DOI: 10.1093/glycob/cwu114ISI: 000350045000003PubMedID: 25335974OAI: oai:DiVA.org:uu-248468DiVA: diva2:799355
Available from: 2015-03-30 Created: 2015-03-30 Last updated: 2017-12-04Bibliographically approved

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Eriksson, IngerKjellén, Lena

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