Mechanistic investigation of the on surface enzymatic digestion (oSED) protein adsorption detection method using targeted mass spectrometry
2016 (English)In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 141, no 5, 1714-1720 p.Article in journal (Refereed) Published
This study describes our efforts to study some of the mechanistic aspects of the earlier established onsurface enzymatic digestion (oSED) method. In a multitude of application areas, it has become important to be able to fully characterize and understand selective protein adsorption to biomaterial surfaces for various applications, including biomedicine (implants), nanotechnology (microchip surfaces and sensors) and materials sciences. Herein, the investigation of the mechanistic aspects was based on microdialysis catheter tubes that were flushed with controlled protein solutions mimicking the extracellular fluid of the brain. The protein adsorption properties were monitored using high-resolution liquid chromatography tandem mass spectrometry (LC-MS/MS) with a targeted method. The temporally resolved results show that most proteins stay adsorbed onto the surface during the entire digestion process and are only cut away piece by piece, whereas smaller proteins and peptides seem to desorb rather easily from the surface. This information will simplify the interpretation of data generated using the oSED method and can also be used for the characterization of the physicochemical properties controlling the adsorption of individual proteins to specific surfaces.
Place, publisher, year, edition, pages
2016. Vol. 141, no 5, 1714-1720 p.
Analytical Chemistry Engineering and Technology
Research subject Chemistry with specialization in Analytical Chemistry
IdentifiersURN: urn:nbn:se:uu:diva-248629DOI: 10.1039/c5an02091cISI: 000371229600018OAI: oai:DiVA.org:uu-248629DiVA: diva2:800460
FunderMagnus Bergvall FoundationBerzelii Centre EXSELENTSwedish Research Council, 621-2011-4423