uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Vascular dysfunction and increased metastasis of B16F10 melanomas in Shb deficient mice as compared with their wild type counterparts
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Karoliska Institutet.
Show others and affiliations
2015 (English)In: BMC Cancer, ISSN 1471-2407, E-ISSN 1471-2407, Vol. 15, 234Article in journal (Refereed) Published
Abstract [en]

Background Shb is a signaling protein downstream of vascular endothelial growth factor receptor-2 and Shb deficiency has been found to restrict tumor angiogenesis. The present study was performed in order to assess metastasis in Shb deficiency using B16F10 melanoma cells. Methods B16F10 melanoma cells were inoculated subcutaneously on wild type or Shb +/− mice. Primary tumors were resected and lung metastasis determined after tumor relapse. Lung metastasis was also assessed after bone marrow transplantation of wild type bone marrow to Shb +/− recipients and Shb +/− bone marrow to wild type recipients. Primary tumors were subject to immunofluorescence staining for CD31, VE-cadherin, desmin and CD8, RNA isolation and isolation of vascular fragments for further RNA isolation. RNA was used for real-time RT-PCR and microarray analysis. Results Numbers of lung metastases were increased in Shb +/− or −/− mice and this coincided with reduced pericyte coverage and increased vascular permeability. Gene expression profiling of vascular fragments isolated from primary tumors and total tumor RNA revealed decreased expression of different markers for cytotoxic T cells in tumors grown on Shb +/− mice, suggesting that vascular aberrations caused altered immune responses. Conclusions It is concluded that a unique combinatorial response of increased vascular permeability and reduced recruitment of cytotoxic CD8+ cells occurs as a consequence of Shb deficiency in B16F10 melanomas. These changes may promote tumor cell intravasation and metastasis.

Place, publisher, year, edition, pages
2015. Vol. 15, 234
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-248693DOI: 10.1186/s12885-015-1269-yISI: 000352603200001PubMedID: 25885274OAI: oai:DiVA.org:uu-248693DiVA: diva2:800741
Available from: 2015-04-07 Created: 2015-04-07 Last updated: 2017-12-04Bibliographically approved

Open Access in DiVA

fulltext(2567 kB)194 downloads
File information
File name FULLTEXT01.pdfFile size 2567 kBChecksum SHA-512
5bfb15b0f71be3ff68c8a109ac3a0cc61b4712453e3c66f608ea7ea0b8e69a9f83c5e708352fc9ac2ca457b41111e4bb1b7ba02561cf3401da3bfb8f47765f84
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Gustafsson, KarinJamalpour, MariaWelsh, Michael

Search in DiVA

By author/editor
Gustafsson, KarinJamalpour, MariaWelsh, Michael
By organisation
Department of Medical Cell Biology
In the same journal
BMC Cancer
Cell and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 194 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 642 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf