uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Ontogenetic Characterization of Müllerian Duct Development and Expression of anti-Müllerian hormone and Other Genes: New Endpoints in the Frog Xenopus tropicalis Test System
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Environmental toxicology.
2015 (English)Licentiate thesis, comprehensive summary (Other academic)
Abstract [en]

The amphibian model-species Xenopus tropicalis is used in reproductive toxicology studies. Lifecycle studies are requested but they are time consuming and susceptible to several of the risks attributed to long-term studies. Herein, the ontogenetic expression of genes associated to gonads and Müllerian ducts and the early Müllerian duct development were characterized as well as explored as suitable early juvenile endpoints after developmental levonorgestrel (LNG) exposure. The urogenital mRNA expression of the anti-Müllerian hormone (amh) was male-biased while the cytochrome P450 19a1 (cyp19a1) mRNA expression was female-biased from the Nieuwkoop and Faber developmental stage (NF) 50, to 4 weeks post-metamorphosis. amh expression in combination with cyp19a1 expression is proposed to constitute a robust marker of phenotypic sex during gonadal differentiation in amphibians. The expression of the amh receptor 2 (amhr2) was characterized for the first time in amphibians. amhr2 was expressed higher in putative females than in males from NF 56, suggesting a role in ovarian development. The mRNA levels of intracellular and membrane progesterone receptors (ipgr and mpgr beta) respectively increased and decreased as the tadpole developed into a juvenile frog, indicating different roles for these genes during development. Exposure to LNG modulated the mRNA expression of the amh and pgrs in a sex and developmental stage dependent manner, suggesting the involvement of these genes in the LNG mode of action. The Müllerian ducts were first observed at NF 64 in both sexes and grew into distinct tubules, larger in females than in males at 4 weeks post-metamorphosis. Therefore, 4 weeks post-metamorphosis is suggested as a suitable early juvenile stage for investigation into disruptions of female reproductive organ development. In conclusion, the protein Amh is suggested to be involved in testicular differentiation and to be a reliable testicular marker during gonadal differentiation, even after developmental LNG exposure, in amphibians.

Place, publisher, year, edition, pages
Uppsala: Uppsala universitet, 2015.
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-248785OAI: oai:DiVA.org:uu-248785DiVA: diva2:800983
Supervisors
Funder
Mistra - The Swedish Foundation for Strategic Environmental ResearchSwedish Research Council Formas
Available from: 2015-04-20 Created: 2015-04-08 Last updated: 2015-04-20Bibliographically approved
List of papers
1. Sex dependent ontogenetic expression of anti-Müllerian hormone (amh) and amh receptor 2 in Xenopus (Silurana) tropicalis
Open this publication in new window or tab >>Sex dependent ontogenetic expression of anti-Müllerian hormone (amh) and amh receptor 2 in Xenopus (Silurana) tropicalis
(English)Article in journal (Other academic) Submitted
National Category
Biological Sciences
Identifiers
urn:nbn:se:uu:diva-248758 (URN)
Available from: 2015-04-08 Created: 2015-04-08 Last updated: 2015-04-20
2. Levonorgestrel up-regulates anti-Müllerian hormone (amh) and progesterone receptors (pgrs) mRNA expression during sex organ differentiation in Xenopus (Silurana) tropicalis
Open this publication in new window or tab >>Levonorgestrel up-regulates anti-Müllerian hormone (amh) and progesterone receptors (pgrs) mRNA expression during sex organ differentiation in Xenopus (Silurana) tropicalis
(English)Manuscript (preprint) (Other academic)
National Category
Biological Sciences
Identifiers
urn:nbn:se:uu:diva-248779 (URN)
Available from: 2015-04-08 Created: 2015-04-08 Last updated: 2015-04-20

Open Access in DiVA

No full text

Authority records BETA

Jansson, Erika

Search in DiVA

By author/editor
Jansson, Erika
By organisation
Environmental toxicology
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 940 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf