High resolution genetic typing of the class II HLA-DRB1 locus using group-specific amplification and SSO-hybridisation in microplates
1998 (English)In: Hereditas, ISSN 0018-0661, E-ISSN 1601-5223, Vol. 129, no 2, 161-167 p.Article in journal (Refereed) Published
The HLA-DRB1 locus is one of the most polymorphic HLA class II loci and rapid and accurate typing of this polymorphism is important both in bone-marrow transplantation, analysis of disease association and in forensic medicine. The allelic variation at DRB1 is characterized by combinations of a limited number of amino-acid motifs, reducing the resolution of a typing strategy based on a single PCR and subsequent analysis of polymorphic motifs. In the present paper we describe a strategy for typing of DRB1 based on eight allele-specific PCRs followed by sandwich hybridization to immobilized probes in a microplate format. The combined approach results in a rapid typing system with very high resolution. Using a rapid DNA extraction protocol, a complete HLA-DRB1 typing can be performed in less than a day.
Place, publisher, year, edition, pages
1998. Vol. 129, no 2, 161-167 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-52277DOI: 10.1111/j.1601-5223.1998.00161.xPubMedID: 10022082OAI: oai:DiVA.org:uu-52277DiVA: diva2:80186