Inflammatory response to nano- and microstructured hydroxyapatite
2015 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 10, no 4, e0120381Article in journal (Refereed) Published
The proliferation and activation of leukocytes upon contact with a biomaterial play a crucial role in the degree of inflammatory response, which may then determine the clinical failure or success of an implanted biomaterial. The aim of this study was to evaluate whether nano- and microstructured biomimetic hydroxyapatite substrates can influence the growth and activation of macrophage-like cells. Hydroxyapatite substrates with different crystal mor- phologies consisting of an entangled network of plate-like and needle-like crystals were evaluated. Macrophage proliferation was evaluated on the material surface (direct contact) and also in extracts i.e. media modified by the material (indirect contact). Additionally, the ef- fect of supplementing the extracts with calcium ions and/or proteins was investigated. Mac- rophage activation on the substrates was evaluated by quantifying the release of reactive oxygen species and by morphological observations. The results showed that differences in the substrate’s microstructure play a major role in the activation of macrophages as there was a higher release of reactive oxygen species after culturing the macrophages on plate- like crystals substrates compared to the almost non-existent release on needle-like sub- strates. However, the difference in macrophage proliferation was ascribed to different ionic exchanges and protein adsorption/retention from the substrates rather than to the texture of materials.
Place, publisher, year, edition, pages
2015. Vol. 10, no 4, e0120381
Other Materials Engineering
Research subject Engineering Science with specialization in Materials Science
IdentifiersURN: urn:nbn:se:uu:diva-249169DOI: 10.1371/journal.pone.0120381ISI: 000352139000020PubMedID: 25837264OAI: oai:DiVA.org:uu-249169DiVA: diva2:802179