uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Nanoconfined Circular and Linear DNA: Equilibrium Conformations and Unfolding Kinetics
Show others and affiliations
2015 (English)In: Macromolecules, ISSN 0024-9297, E-ISSN 1520-5835, Vol. 48, no 3, 871-878 p.Article in journal (Refereed) Published
Abstract [en]

Studies of circular DNA confined to nanofluidic channels are relevant both from a fundamental polymer-physics perspective and due to the importance of circular DNA molecules in vivo. We here observe the unfolding of confined DNA from the circular to linear configuration as a light-induced double-strand break occurs, characterize the dynamics, and compare the equilibrium conformational statistics of linear and circular configurations. This is important because it allows us to determine to what extent existing statistical theories describe the extension of confined circular DNA. We find that the ratio of the extensions of confined linear and circular DNA configurations increases as the buffer concentration decreases. The experimental results fall between theoretical predictions for the extended de Gennes regime at weaker confinement and the Odijk regime at stronger confinement. We show that it is possible to directly distinguish between circular and linear DNA molecules by measuring the emission intensity from the DNA. Finally, we determine the rate of unfolding and show that this rate is larger for more confined DNA, possibly reflecting the corresponding larger difference in entropy between the circular and linear configurations.

Place, publisher, year, edition, pages
2015. Vol. 48, no 3, 871-878 p.
National Category
Cell Biology Biochemistry and Molecular Biology
URN: urn:nbn:se:uu:diva-248190DOI: 10.1021/ma5022067ISI: 000349574100046OAI: oai:DiVA.org:uu-248190DiVA: diva2:802319
Available from: 2015-04-12 Created: 2015-03-30 Last updated: 2015-04-12Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text
By organisation
Department of Cell and Molecular Biology
In the same journal
Cell BiologyBiochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 581 hits
ReferencesLink to record
Permanent link

Direct link