The adenovirus-2 E1B-55K protein interacts with a mSin3A/histone deacetylase 1 complex
2000 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 476, no 3, 248-252 p.Article in journal (Refereed) Published
The adenovirus E1B-55K protein is a multifunctional phosphoprotein that regulates nuclear to cytoplasmic export of host cell and viral mRNAs during lytic viral growth. E1B-55K also blocks apoptosis by binding and functionally inactivating the human tumor suppressor protein p53. Here, we show that E1B-55K interacts with histone deacetylase 1 (HDAC1) and the transcriptional corepressor protein mSin3A, both in the adenovirus-transformed 293 cell line and during a lytic adenovirus infection. Furthermore, we show that the central amino acids 156-261 in E1B-55K are necessary for efficient HDAC1 interaction. Importantly, the E1B-55K/mSin3A/HDAC1 complex is also enzymatically active, catalyzing deacetylation of a histone substrate peptide. Collectively, our results suggest that E1B-55K interaction with mSin3A/HDAC1 containing complexes may be significant for one or several of the multiple activities ascribed to this protein.
Place, publisher, year, edition, pages
2000. Vol. 476, no 3, 248-252 p.
Adenovirus, E1B-55K, Histone deacetylase 1, mSin3A
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-52394DOI: 10.1016/S0014-5793(00)01739-7ISI: 000088245000027PubMedID: 10913622OAI: oai:DiVA.org:uu-52394DiVA: diva2:80303