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Hgf/sf induces mesothelial cell migration and proliferation by autocrineand paracrine pathways.
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2001 (English)In: Exp Cell Res, Vol. 267, 258- p.Article in journal (Refereed) Published
Abstract [en]

Mesothelial repair differs from that of other epithelial-like surfaces as healing does not occur solely by centripetal in-growth of cells as a sheet from the wound margins. Mesothelial cells lose their cell-cell junctions, divide, and adopt a fibroblast-like morphology while scattering across and covering the wound surface. These features are consistent with a cellular response to hepatocyte growth factor/scatter factor (HGF/SF). In this study, we examined the ability of mesothelial cells to secrete HGF/SF and investigated its possible role as an autocrine regulator of mesothelial cell motility and proliferation. We found that human primary mesothelial cells expressed HGF/SF mRNA and secreted active HGF/SF into conditioned medium as determined by ELISA and in a scattering bioassay. These cells also expressed the HGF/SF receptor, Met, as shown by RT-PCR and by Western blot analysis and immunofluorescence. Incubation of mesothelial cells with neutralizing antibodies to HGF/SF decreased cell migration to 25% of controls, whereas addition of HGF/SF disrupted cell-cell junctions and induced scattering and enhanced mesothelial cell migration. Furthermore, HGF/SF showed a small but significant mitogenic effect on all mesothelial cell lines examined. In conclusion, HGF/SF is produced by mesothelial cells and induces both motility and proliferation of these cells. These data are consistent with HGF/SF playing an autocrine role in mesothelial healing.

Place, publisher, year, edition, pages
2001. Vol. 267, 258- p.
Identifiers
URN: urn:nbn:se:uu:diva-52460PubMedID: 11426944OAI: oai:DiVA.org:uu-52460DiVA: diva2:80369
Available from: 2005-06-09 Created: 2005-06-09 Last updated: 2011-01-14

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Heldin, Paraskevi

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