uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Hgf/sf induces mesothelial cell migration and proliferation by autocrineand paracrine pathways.
Show others and affiliations
2001 (English)In: Exp Cell Res, Vol. 267, 258- p.Article in journal (Refereed) Published
Abstract [en]

Mesothelial repair differs from that of other epithelial-like surfaces as healing does not occur solely by centripetal in-growth of cells as a sheet from the wound margins. Mesothelial cells lose their cell-cell junctions, divide, and adopt a fibroblast-like morphology while scattering across and covering the wound surface. These features are consistent with a cellular response to hepatocyte growth factor/scatter factor (HGF/SF). In this study, we examined the ability of mesothelial cells to secrete HGF/SF and investigated its possible role as an autocrine regulator of mesothelial cell motility and proliferation. We found that human primary mesothelial cells expressed HGF/SF mRNA and secreted active HGF/SF into conditioned medium as determined by ELISA and in a scattering bioassay. These cells also expressed the HGF/SF receptor, Met, as shown by RT-PCR and by Western blot analysis and immunofluorescence. Incubation of mesothelial cells with neutralizing antibodies to HGF/SF decreased cell migration to 25% of controls, whereas addition of HGF/SF disrupted cell-cell junctions and induced scattering and enhanced mesothelial cell migration. Furthermore, HGF/SF showed a small but significant mitogenic effect on all mesothelial cell lines examined. In conclusion, HGF/SF is produced by mesothelial cells and induces both motility and proliferation of these cells. These data are consistent with HGF/SF playing an autocrine role in mesothelial healing.

Place, publisher, year, edition, pages
2001. Vol. 267, 258- p.
URN: urn:nbn:se:uu:diva-52460PubMedID: 11426944OAI: oai:DiVA.org:uu-52460DiVA: diva2:80369
Available from: 2005-06-09 Created: 2005-06-09 Last updated: 2011-01-14

Open Access in DiVA

No full text


Search in DiVA

By author/editor
Heldin, Paraskevi
By organisation
Department of Medical Biochemistry and Microbiology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 146 hits
ReferencesLink to record
Permanent link

Direct link