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A downstream splicing enhancer is essential for in vitro pre-mRNA splicing
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
1999 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 451, no 1, 10-14 p.Article in journal (Refereed) Published
Abstract [en]

Splicing enhancers have previously been shown to promote processing of introns containing weak splicing signals. Here, we extend these studies by showing that also 'strong' constitutively active introns are absolutely dependent on a downstream splicing enhancer for activity in vitro. SR protein binding to exonic enhancer elements or U1 snRNP binding to a downstream 5' splice site serve redundant functions as activators of splicing. We further show that a 5' splice site is most effective as an enhancer of splicing. Thus, a 5' splice site is functional in S100 extracts, under conditions where a SR enhancer is nonfunctional. Also, splice site pairing occurs efficiently in the absence of exonic SR enhancers, emphasizing the significance of a downstream 5' splice site as the enhancer element in vertebrate splicing.

Place, publisher, year, edition, pages
1999. Vol. 451, no 1, 10-14 p.
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Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-52493DOI: 10.1016/S0014-5793(99)00521-9PubMedID: 10356974OAI: oai:DiVA.org:uu-52493DiVA: diva2:80402
Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2017-12-04Bibliographically approved

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Akusjärvi, Göran

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