Two Novel Adenovirus Vector Systems Permitting Regulated Protein Expression in Gene Transfer Experiments
1998 (English)In: Journal of Virology, ISSN 0022-538X, E-ISSN 1098-5514, Vol. 72, no 10, 8358-8861 p.Article in journal (Refereed) Published
Two new adenovirus vector systems based on the tetracycline-regulated Tet-ON- (Gossen, M., et al., Science 268:1766-1769, 1995) and the RU 486-regulated progesterone antagonist (Wang, Y., et al., Proc. Natl. Acad. Sci. USA 91:8180-8184, 1994)-induced gene expression systems are described. We show that both systems permit a tight control of chloramphenicol acetyltransferase reporter gene expression in a variety of cell types, with induction levels of approximately 1,800-fold (Tet-ON system) and 600-fold (RU 486-regulated system), respectively. A significant advantage of our vector systems is that reporter protein expression can be adjusted over a wide range by varying the amount of inducer. The Tet-ON system is also shown to permit an efficient control of reporter gene expression in mice.
Place, publisher, year, edition, pages
1998. Vol. 72, no 10, 8358-8861 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-52578ISI: 000075864100079PubMedID: 9733884OAI: oai:DiVA.org:uu-52578DiVA: diva2:80488