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Splicing of two weak 3' splice sites from the adenovirus major late region is enhanced innuclear extracts from adenovirus infected cells
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
1995 (English)In: Nucleic Acids Symposium Series, ISSN 0261-3166, no 33, 220-223 p.Article in journal (Refereed) Published
Abstract [en]

The adenovirus major late transcription unit (MLTU) is an example of a complex alternatively spliced gene, in which more than 15 different 3' splice sites can be joined to a common 5' splice site. Maturation of the full repertoire of possible mRNAs requires late viral protein synthesis and occurs only at late stages of the infectious cycle (16-24 hpi). We are trying to decipher the mechanisms regulating alternative 3' splice site choice during the infectious cycle. Therefore, we examined the splicing activity of several 3' splice sites from the MLTU in vitro in nuclear extracts prepared from adenovirus infected cells (Ad NE) and from uninfected cells. The results suggest that pre-mRNAs with "weak" 3' splice sites (short, atypical polypyrimidine tracts) are activated and pre-mRNAs with long, prototypical polypyrimidine tracts are repressed in Ad NE. In fact, our data show a reciprocal correlation between the strength of a polypyrimidine tract, defined by its affinity for U2AF65K in vitro, and the efficiency of splicing in Ad NE. We are currently investigating the possible mechanisms responsible for this observed shift in 3' splice site choice during an adenovirus infection.

Place, publisher, year, edition, pages
1995. no 33, 220-223 p.
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:uu:diva-52609PubMedID: 8643376OAI: oai:DiVA.org:uu-52609DiVA: diva2:80519
Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2011-07-22Bibliographically approved

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