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Mode of Interaction Between Pseudorabies Virus and Heparan Sulfate/Heparin
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
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1996 (English)In: Virology, ISSN 0042-6822, E-ISSN 1096-0341, Vol. 218, no 1, 35-42 p.Article in journal (Refereed) Published
Abstract [en]

It has been demonstrated that the efficient attachment of pseudorabies virus (PrV) is mediated by an interaction between glycoprotein C (gC) and a cellular heparin-like substance (T. C. Mettenleiter, L. Zsak, F. Zuckermann, N. Sugg, H. Kern, and T. Ben-Porat, J. Virol. 64, 278–286, 1990). According to the prevalent concept, this interaction is likely to occur between clusters of basic residues of PrV gC and the negatively charged sulfate esters and carboxylate groups of heparan sulfate/heparin. To elucidate which of the three major types of sulfate groups of heparan sulfate/heparin are involved in the interaction with PrV, we used selectively N-, 2-O-, and 6-O-desulfated samples and other modified heparins as competitors in virus-attachment assays. PrV exhibited limited preference for the specific sulfate groups of heparan sulfate/heparin in accordance with a hierarchy of 6-O- > 2-O- >N-sulfates. In addition, since selective removal of any of the specific sulfates had only a slight effect on the competition capacity of heparin, it is likely that the combination of any two of three types of sulfate groups could contribute to an interaction with PrV with an efficiency nearly equal to native, fully sulfated heparin. When tested on different cell lines the pattern of PrV requirement for the specificO-sulfate groups, i.e., 6-O-sulfates > 2-O-sulfates, remained the same. However, different minimum lengths of heparin fragments were required to inhibit PrV attachment to different cell lines, suggesting a relative virus flexibility in accommodation to different forms of heparan sulfate.

Place, publisher, year, edition, pages
1996. Vol. 218, no 1, 35-42 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-52624DOI: 10.1006/viro.1996.0163OAI: oai:DiVA.org:uu-52624DiVA: diva2:80534
Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2011-07-19Bibliographically approved

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Spillmann, Dorothe
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