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Role of the Bsk/lyk non-receptor tyrosine kinase for the control of growth and hormone production in RINm5F cells
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
2000 (English)In: Growth Factors, ISSN 0897-7194, E-ISSN 1029-2292, Vol. 17, 233-247 p.Article in journal (Refereed) Published
Abstract [en]

Bsk/Iyk, a murine non-receptor-tyrosine kinase which is expressed in fetal and adult islet of Langerhans was previously found to decrease NIH3T3 cell proliferation when expressed as a Y497/504F-mutant. We presently wanted to determine the effects of Bsk/Iyk on the proliferation of insulin producing cells. Cells expressing Bsk/IykY497/504F and Bsk/IykY504F display a decreased proliferation rate and express higher levels of the cell cycle inhibitor p27/Kip1 compared to control cells. These mutants also conferred diminished cell viability in response to INF-gamma and IL-1beta and contain higher levels of glucagon mRNA. Wild-type Bsk/Iyk is mainly localized at the plasma membrane whereas mutant Bsk/Iyk can enter the nucleus. In vitro kinase reactions using an exogenous substrate indicate a complicated mode of regulation of kinase activity by Y497 and Y504 with the latter being homologous to Y527 in pp60c-Src. These findings suggest that Bsk/Iyk might play a role in inhibiting cell proliferation, transducing cytokine-induced cytotoxicity and regulating hormone production of endocrine pancreatic cells.

Place, publisher, year, edition, pages
2000. Vol. 17, 233-247 p.
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Medical and Health Sciences
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URN: urn:nbn:se:uu:diva-52820PubMedID: 10801074OAI: oai:DiVA.org:uu-52820DiVA: diva2:80730
Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2017-12-04

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Annerén, CeciliaWelsh, Michael

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