uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Glucose induces oscillations of cytoplasmic Ca2+, Sr2+ and Ba2+ in pancreatic beta-cells without participation of the thapsigargin-sensitive store
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
1995 (English)In: Cell Calcium, Vol. 18, no 2, 165-173 p.Article in journal (Refereed) Published
Abstract [en]

Individual pancreatic beta -cells were used to study the glucose effects on the handling of Ca2+, Sr2+ and Ba2+. In extracellular medium containing one of these ions, single beta -cells responded to 11 mM glucose with large amplitude oscillations in cytoplasmic Ca2+, Sr2+ or Ba2+ with indistinguishable average frequencies (0.30-0.33/min). The oscillations disappeared after hyperpolarization with 400 microM diazoxide. Under such hyperpolarization, glucose stimulated the sequestration of Ca2+ and Sr2+ but not of repetitively mobilized by consecutive exposures to 100 microM carbachol. A 2-3 min exposure to 100 nM of the intracellular Ca(2+)-ATPase inhibitor thapsigargin also mobilized Ca2+ and Sr2+ and irreversibly abolished subsequent release by carbachol. However, thapsigargin did not prevent the large amplitude oscillations in Ca2+, Sr2+ or Ba2+ under non-hyperpolarizing conditions although the frequency of the Ca2+ oscillations was almost doubled. The results indicate that the slow oscillatory behavior of glucose-stimulated individual beta -cells does not depend on inositol 1,4,5-trisphosphate mediated release of intracellular Ca2+.

Place, publisher, year, edition, pages
1995. Vol. 18, no 2, 165-173 p.
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-52938PubMedID: 7585893OAI: oai:DiVA.org:uu-52938DiVA: diva2:80848
Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2013-11-20Bibliographically approved

Open Access in DiVA

No full text

PubMed

Authority records BETA

Grapengiesser, EvaGylfe, ErikHellman, Bo

Search in DiVA

By author/editor
Grapengiesser, EvaGylfe, ErikHellman, Bo
By organisation
Department of Medical Cell Biology
Cell and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

pubmed
urn-nbn

Altmetric score

pubmed
urn-nbn
Total: 624 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf