Fluctuating expression of microRNAs in adenovirus infected cells
2015 (English)In: Virology, ISSN 0042-6822, E-ISSN 1096-0341, Vol. 478, 99-111 p.Article in journal (Refereed) Published
The changes in cellular microRNA (miRNA) expression during the course of an adenovirus type 2 infection in human lung fibroblast were studied by deep RNA sequencing. Expressions of 175 miRNAs with over 100 transcripts per million nucleotides were changed more than 1.5-fold. The expression patterns of these miRNAs changed dramatically during the course of the infection, from upregulation of the miRNAs known as tumor suppressors (such as miR-22, miR-320, let-7, miR-181b, and miR-155) and down-regulation of oncogenic miRNAs (such as miR-21 and miR-31) early to downregulation of tumor suppressor miRNAs (such as let-7 family, mir-30 family, 23/27 cluster) and upregulation of oncogenic miRNAs (include miR-125, miR-27, miR-191) late after infection. The switch in miRNA expression pattern occurred when adenovirus DNA replication started. Furthermore, deregulation of cellular miRNA expression was a step-wise and special sets of miRNAs were deregulated in different phases of infection.
Place, publisher, year, edition, pages
2015. Vol. 478, 99-111 p.
Adenovirus infection, Cellular microRNA, Deep sequencing, Expression profile
IdentifiersURN: urn:nbn:se:uu:diva-251992DOI: 10.1016/j.virol.2015.01.033ISI: 000352116800010PubMedID: 25744056OAI: oai:DiVA.org:uu-251992DiVA: diva2:811123