uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Changes in TDP-43 expression in development, aging, and in the neurofilament light protein knockout mouse
Show others and affiliations
2015 (English)In: Neurobiology of Aging, ISSN 0197-4580, E-ISSN 1558-1497, Vol. 36, no 2, 1151-1159 p.Article in journal (Refereed) Published
Abstract [en]

The transactive response DNA-binding protein 43 (TDP-43) has been identified as a neurofilament light (NF-L) messenger RNA (mRNA)-binding protein. Abnormally increased levels of TDP-43 are detected in patients with amyotrophic lateral sclerosis and a downregulation of NF-L mRNA. However, links between NF-L and TDP-43 expressions are unclear. In this study, we investigated whether the deficiency of NF-L protein can result in alterations in TDP-43 localization or protein expression and whether this is altered with aging. There was a significant increase in TDP-43 protein levels in the cortex and lumbar spinal cord in 12-month-old NF-L knockout (NF-L KO) mice, compared with wild-type (WT) C57BL/6 mice. However, there was no difference in either the phosphorylation of TDP-43 between WT and NF-L KO mice or the abnormal mislocalization of TDP-43 to the cytoplasm in NF-L KO animals. Our findings suggest that NF-L protein or mRNA may negatively affect the expression of TDP-43 in the central nervous system. However, altered phosphorylation of TDP-43 may be more highly associated with aging than the levels of TDP-43 expression.

Place, publisher, year, edition, pages
2015. Vol. 36, no 2, 1151-1159 p.
Keyword [en]
Transactive response DNA-binding protein 43 (TDP-43), Neurofilament light (NF-L), Amyotrophic lateral sclerosis (ALS), Phosphorylated TDP-43
National Category
Geriatrics Neurosciences
URN: urn:nbn:se:uu:diva-252731DOI: 10.1016/j.neurobiolaging.2014.10.001ISI: 000352454400061PubMedID: 25457553OAI: oai:DiVA.org:uu-252731DiVA: diva2:811429
Available from: 2015-05-12 Created: 2015-05-11 Last updated: 2015-05-12Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed
By organisation
Department of Medical Biochemistry and Microbiology
In the same journal
Neurobiology of Aging

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 276 hits
ReferencesLink to record
Permanent link

Direct link