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Ethanol production by Zymomonas mobilis PTCC 1718 using low cost substrates
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
2012 (English)In: African Journal of Microbiology Research, ISSN 1996-0808, E-ISSN 1996-0808, Vol. 6, no 4, 704-712 p.Article in journal (Refereed) Published
Abstract [en]

We report our attempts to increase ethanol production from Zymomonas mobilis PTCC 1718, by optimization of the components of growth medium and culture conditions. First, low cost substrates, carbon and nitrogen sources, temperature and initial pH were optimized by one-factor-at-a-time method. Then, nutritional improvement was performed using Taguchi method. Amongst the different selected sources, sucrose and wheat bran have significant effects on ethanol production. The optimum temperature, inoculum-size and pH were 50 degrees C, 12% (v/v) and 5.5, respectively. The optimal concentrations of nutritional components for improved ethanol production were. 2.5 g/L sucrose, 1.5 g/L whey, 0.25 g/L wheat bran extract, 0.5 g/L yeast extract, 0.25 g/L peptone, 0.7 g/L KH2PO4, 0.1 g/L (NH4)(2)SO4 and 0.05 g/L MgSO4. Ethanol production, which was 14.49 g/L (w/v) in the basal medium, increased to 41.67 g/L (w/v) and 86.26 g/L (v/v); about 2.8 and 5.9-fold higher than that in the basal medium, after optimization with one-factor-at-a-time and Taguchi method, respectively. Bioreactor fermentation was carried out in a 15 L stirred fermentor under optimal condition. Maximum ethanol production in 15 L fermentor indicated 91.22 g/L (w/v), about 6.3 fold higher than the basal medium.

Place, publisher, year, edition, pages
2012. Vol. 6, no 4, 704-712 p.
Keyword [en]
Ethanol, medium components, Zymomonas mobilis PTCC 1718, optimization, Taguchi method
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-253107DOI: 10.5897/AJMR11.576ISI: 000306480900001OAI: oai:DiVA.org:uu-253107DiVA: diva2:812838
Available from: 2015-05-20 Created: 2015-05-20 Last updated: 2017-12-04Bibliographically approved

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