uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
A Transgenic Prox1-Cre-tdTomato Reporter Mouse for Lymphatic Vessel Research
Show others and affiliations
2015 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 10, no 4, e0122976Article in journal (Refereed) Published
Abstract [en]

The lymphatic vascular system plays an active role in immune cell trafficking, inflammation and cancer spread. In order to provide an in vivo tool to improve our understanding of lymphatic vessel function in physiological and pathological conditions, we generated and characterized a tdTomato reporter mouse and crossed it with a mouse line expressing Cre recombinase under the control of the lymphatic specific promoter Prox1 in an inducible fashion. We found that the tdTomato fluorescent signal recapitulates the expression pattern of Prox1 in lymphatic vessels and other known Prox1-expressing organs. Importantly, tdTomato co-localized with the lymphatic markers Prox1, LYVE-1 and podoplanin as assessed by whole-mount immunofluorescence and FACS analysis. The tdTomato reporter was brighter than a previously established red fluorescent reporter line. We confirmed the applicability of this animal model to intravital microscopy of dendritic cell migration into and within lymphatic vessels, and to fluorescence-activated single cell analysis of lymphatic endothelial cells. Additionally, we were able to describe the early morphological changes of the lymphatic vasculature upon induction of skin inflammation. The Prox1-Cre-tdTomato reporter mouse thus shows great potential for lymphatic research.

Place, publisher, year, edition, pages
2015. Vol. 10, no 4, e0122976
National Category
Medical Genetics
Identifiers
URN: urn:nbn:se:uu:diva-252691DOI: 10.1371/journal.pone.0122976ISI: 000352477800186PubMedID: 25849579OAI: oai:DiVA.org:uu-252691DiVA: diva2:813887
Available from: 2015-05-25 Created: 2015-05-11 Last updated: 2017-12-04Bibliographically approved

Open Access in DiVA

fulltext(12255 kB)205 downloads
File information
File name FULLTEXT01.pdfFile size 12255 kBChecksum SHA-512
904657e8dee21b5056063d3a54fdf3dd9b47ae4a5fc76567899c3bf089a6e102b3091fd8e4426125cec810de2c2bd0860ec050aa40f4aca6f04ac8eced7599a9
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Mäkinen, Taija

Search in DiVA

By author/editor
Mäkinen, Taija
By organisation
Vascular Biology
In the same journal
PLoS ONE
Medical Genetics

Search outside of DiVA

GoogleGoogle Scholar
Total: 205 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 675 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf