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Photoinduced reduction of the medial FeS center in the hydrogenase small subunit HupS from Nostoc punctiforme
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Physical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics. (Microbial chemistry)
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
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2015 (English)In: Journal of Inorganic Biochemistry, ISSN 0162-0134, E-ISSN 1873-3344, Vol. 148, 57-61 p.Article in journal (Refereed) Published
Abstract [en]

The small subunit from the NiFe uptake hydrogenase, HupSL, in the cyanobacterium Nostoc punctiforme ATCC 29133, has been isolated in the absence of the large subunit (P. Raleiras, P. Kellers, P. Lindblad, S. Styring, A. Magnuson, J. Biol. Chem. 288 (2013) 18,345-18,352). Here, we have used flash photolysis to reduce the iron-sulfur clusters in the isolated small subunit, HupS. We used ascorbate as electron donor to the photogenerated excited state of Ru(II)-trisbipyridine (Ru(bpy)3), to generate Ru(I)(bpy)3 as reducing agent. Our results show that the isolated small subunit can be reduced by the Ru(I)(bpy)3 generated through flash photolysis.

Place, publisher, year, edition, pages
2015. Vol. 148, 57-61 p.
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Biochemistry and Molecular Biology
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URN: urn:nbn:se:uu:diva-253857DOI: 10.1016/j.jinorgbio.2015.03.018ISI: 000357702100008PubMedID: 25912316OAI: oai:DiVA.org:uu-253857DiVA: diva2:816287
Available from: 2015-06-03 Created: 2015-06-03 Last updated: 2017-12-04Bibliographically approved

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Raleiras, PatríciaHammarström, LeifLindblad, PeterStyring, StenbjörnMagnuson, Ann

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